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学校编号10394图书分类号
学号20161100密级
全日制学术型研究生硕士学位论文
番茄青枯病植物疫苗构建及其免疫抗病机理研究omatobacterialwiltplantsvaccinbuildingandimmunologicmechanism
张文州学科专业:微生物学
研究方向:植物病害防治
指导教师:黄建忠教授,刘波研究员
申请学位级别:理学硕士论文提交日期:年月日
论文评阅人:
论文答辩日期:年月日
答辩委员会主席:
学位授予单位:福建师范大学
学位授予日期:年月日年月 中文摘 要
番茄青枯病植物疫苗番茄青枯病植物疫苗免疫抗病机理作植物疫苗.工程菌FJAT-1458同时接种工程菌FJAT-1458和菌株FJAT-91,,工程菌FJAT-14583d,6d再接种菌株FJAT-91,番茄青枯病的发病率较高.
2,植物疫苗工程菌FJAT-1458植物疫苗FJAT-1458胁迫接种对番茄植株株高,根系长度,根系干重影响不显着(P>,0.05),但对番茄植株不同部位的抗氧化酶影响较明显.植物疫苗FJAT-1458胁迫接种能导致番茄植株不同器官的POD,PPO和SOD酶活性总和显着高于对照植株,并且在胁迫处理植株与对照植株之间,POD,PPO和SOD的活性变化动态也有所不同.接种植物疫苗FJAT-1458能改变根系土壤微生物种群结构和含量,FJAT-1458胁迫,不同作物根系土壤微生物总量均比对照组高进一步分析表明,接种FJAT-1458菌株能改变根系土壤微生物群落结构,促进细菌和真菌的生长,抑制放线菌的生长.丝瓜芹菜Simpson指数茄子辣椒Simpson指数番茄芹菜Shannon指数茄子,辣椒丝瓜Shannon指数Pielou指数值
关 键 词:植物疫苗,定殖,防御酶系统,土壤微生物,根系提取液Abstract
Thestudyexpoundedtheimmuneresistancemechanismfromreproductivecharacteristics,inducingphysiologicalmechanismofimmunediseaseresistanceoftomatobacterialwiltplantvaccineandmicrobialpopulationstructurechangesofplantrhizospheresoil.Mainfindingswereasfollows:
1,ThestudymadepathogenicitytestandcontrolefficacyevaluationtothedifferentpathogenicRalstoniasolanacearumfromthelaboratorysaved,strain(FJAT-1458)wasfoundusedasaplantvaccineengineeringbacteriafromthem,whichhadthebestcontrolefficacy,largecolonization,longcyclecolonizationandbiologicalcharacteristicssTab..Theresultsshowedthat,thedeeperFJAT-1458vaccinationconcentrationsthelowermorbidityoftomatobacterialwilt.WheninoculatingthestrainsofFJAT-1458andFJAT-91atthesametime,themorbidityoftomatobacterialwiltwasthelowest.WhilestrainsFJAT-1458atthefirst,theninoculatingFJAT-91strainsat3dand6dintervally,themorbiditywashigher.
2,PlantvaccineengineeringbacteriaFJAT-1458couldnotcolonizedintheleafbladeofplantbutthesoil,rootsandstemoftomatorhizospheresoil.AccordingtothedifferentvaccinationconcentrationofplantvaccineengineeringbacteriaFJAT-1458,thecolonizationcontentwasdifferentintomatoplantandrhizospheresoil,whenvaccinationconcentrationwasthehigher,thecolonizationcontentwasmoreandthecolonizationtimewaslonger.
3,TheresultsshowedthattherootlengthanddryweightandtheplantheightoftomatowerenotobviouslyaffectedbyplantvaccineengineeringbacteriaFJAT-1458(P>,0.05),buttheactivitiesofantioxidantenzymesweresignificantlyaffected.ThetotalactivitiesofPOD,PPOandSODatdifferentpartsoftomatoplantintreatmentweresignificantlyhigherthanincontrol.Moreover,theactivitydynamicsofPOD,PPOandSODwerealsosignificantlydifferentbetweentreatmentandcontrol.
4,AccordingtovaccinatingplantvaccineengineeringbacteriaFJAT-1458,themicrobialpopulationstructureandcontentofsoilinrootwerechanged.AftertreatmentingbystrainsFJAT-1458,themicrobialtotalcontentofdifferentcroprootsoilswerehigherthanincontrol,andthegroupschangingofPLFAscouldbedividedintodecliningtype,unchangingtype,increasingtype.ThestatisticalanalysisshowedthattheinoculationofFJAT-1458couldchangethemicrobialmunitystructureinthedifferentcroprhizospheresoilduetoimprovingthegrowthofbacteriaandfungiandinhibitingthereproductionofactinomycetes.TheplantvaccineengineeringbacteriaFJAT-1458treatmentingcouldimprovetheSimpsonexponentvalueoftowelgourd,tomatoes,celeryrhizospheresoil,reducingSimpsonexponentvalueoftheeggplantandchilirhizospheresoil,AndwhichalsocouldimproveShannonexponentvalueofthetomatoesandceleryrhizospheresoil,reducingShannonexponentvalueoftheeggplant,peppersandtowelgourdrhizospheresoil,FJAT-1458couldreducePielouexponentvalueofthese5kindsofcroprhizospheresoil.
5,DifferentrhizosphereextractshadthedifferenteffectoftheplantsvaccineengineeringbacteriaFJAT-1458growthability.Therhizosphereextractsoftomato,pepperanddwarfbananacouldpromotestrainFJAT-1458growthing,whiletheleekrhizosphereextractsinhibitedstrainFJAT-1458growthing.Throughspectroscopyscanninganalysisshowedthat,afertreatmenting32hoursofrhizosphereextractsfrompepperanddwarfbanana,nucleicacidandproteincharacteristicabsorptionpeakin260nmand280nmplacewerefailedtofind,whileaabsorptionshoulderpeakwasfoundinthe250-300nmsectionoftomatoeandleek.TheresultsshowedthatthecolonymorphologyandattenuationindexofplantsvaccineengineeringbacteriaFJAT-1458werenotobviouslydifferentbydifferentrhizosphereextracts.
Keywords:plantvaccine,colonization,protectiveenzymessystem,soilmicroanism,rhizosphereextract
中文文摘
细菌性青枯病是一种由青枯雷尔氏菌引起的病害,该菌寄主广,54个科的450余种物,严重影响了多重要经济作物,对农业造成重大的经济损失.近年来广泛受到植物病研究者的重视.
无致病力青枯雷尔氏菌诱导植株产生抗性达到免疫保护作用方面的研究相对较少.无致病力青枯雷尔氏菌诱导植株抗病机制研究,对了解病原菌与植株的互作方式,阐明植株系统性获得抗性的机制,提高青枯病生物防治
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