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生物医学方面有关论文范文资料,与现代生物医学进展相关论文目录怎么自动生成

本论文是一篇生物医学方面有关论文目录怎么自动生成,关于现代生物医学进展相关硕士学位毕业论文范文。免费优秀的关于生物医学及数据库及参考文献方面论文范文资料,适合生物医学论文写作的大学硕士及本科毕业论文开题报告范文和学术职称论文参考文献下载。

培养基+1%PS的培养液.在液氮罐中取出HepG2冻存管,迅速放入37℃水浴箱中复温解冻,在超净台中吸取10ml培养液加入离心管中,待HepG2解冻后,用吸管吸出解冻液,加入离心管中,吹打混匀,密封后置离心机中离心(800r/min,5min),弃上清,加入配制好的培养液适当稀释,移入培养瓶中,于37℃5%CO2培养箱中常规培养.隔天换液,当HepG2生长融合成单层时,用PBS洗涤三遍,再用0.25%胰酶-0.02%EDTA消化传代.

1.分组传代好的HepG2分组浓度为0mmol/L对照组1.0mmol/L,2.0mmol/L,4.0mmol/L,8.0mmol/L(乳酸1.0mmol/L+DCA,2.0mmol/L+DCA,4.0mmol/L+DCA,8.0mmol/L+DCA(DCA组),DCA终浓度为10-3mmol/L培养液共同培养24.

1.2.3四氮唑蓝(MTT)比色实验将HePG2细胞悬液浓度调整为5×l07/L,每孔200μL接种于96孔板上,各组分别加入不同浓度的乳酸,对照组不加药,另设空白对照孔只加培养基,无细胞,每组设8个重复孔.继续培养24后,加人MTT5g/L)20μL,放置孵箱内培养4h后弃上清,加人二甲基亚矾DMSO)200μL,振荡15min,用全自动酶标仪BIO-RAD550)检测490nm参考波长630nm处的吸光度值A值.抑制率%)等于(A对照-A实验/A对照×100%.

1.2.4流式细胞仪测定凋亡取各组细胞,分别用0.25%胰酶-0.02%EDTA消化30s,PBS洗涤后加入配制好的培养液制备成单细胞悬液,用细胞计数器调整每组细胞数为5×106/ml,取1ml细胞悬液于离心管中,置于离心机中800r/min离心5min,弃上清,PBS洗涤后轻轻振荡使细胞悬浮,反复离心洗涤后,取250μL细胞悬液,加入AnnexinV-FITC10μL和PI5μL,混匀后室温下避光孵育15min后上机待检.

1.统计学分析

研究型论文必须有统计学分析,注明所采用的统计学软件及版本号,检验方法及检验水准等.

2结果

应详细说明研究得到的真实数据或观察到的实验现象,并辅以图或表补充说明.

例:

2.1各组大鼠心肌细胞凋亡情况的比较

模型组大鼠AMI出现大量区心肌细胞凋亡凋亡细胞数显着多于假手术组P<,0.001),而OMT预处理使凋亡数显着减少P<,0.001),在组与OMT预处理假手术组中仅发现极少量的凋亡细胞存在.图

图1各组大鼠心肌细胞凋亡情况的比较

Fig.1Comparisonoftheapoptosisofratmyocardialcellsamongdifferentgroups

Note:Datawereexpressedas±SD,n等于6.***P<,0.001,paredwithgroupSham,###P<,0.001,paredwithAMIgroup.

例:

2.2胃癌细胞SGC7901的凋亡情况

流式细胞仪检测肿瘤细胞凋亡结果显示治疗组,阴性对照组及对照组胃癌细胞凋亡率分别为(22.28±0.12)%,(1.23±0.17)%和(1.03±0.14)%.治疗组与阴性对照组和对照组比较差异都有统计学意义t等于18.152,17.555,P<,0.05.见表1.

表1三组胃癌SGC7901细胞凋亡情况(%,x±s)

Table1ApoptosisastriccancerSGC7901ofthethreegroups

GroupsAmount(n)ApoptosisRateTreatmentGroup822.28±0.12#*NegativeControlGroup81.23±0.17ControlGroups81.03±0.14Note:paredwiththenegativegroup,#t等于18.152,paredwiththecontrolgroup,*t等于17.555,P<,0.05.

3讨论

对研究结果进行具体的总结应准确,简明,完整的分析各指标的意义,评价结果存在的误差,结合相关文献进行比较,说明结果产生的可能原因,强调本研究对该领域的意义以及后续研究需要注意的方面等.简要介绍下一步研究思路,进一步提出新问题,并对该领域的研究进行展望.

参考文献(References)(五号体&,TimesNewRoman加粗)

例:

[1]LiH,CaoW,XuY,etal.InflammatorypaininducedCBPexpressionchangeinrathippocampus[J].ChineseJournalofClinicalAnatomy(中国临床解剖学杂志,2016,29(5):546-549

[2]MalheirosJM,LimaM,AvanziRD,etal.Repetitivenoxiousneonatalstimuliincreasesdentategyruscellproliferationandhippocampalbrain-derivedneurotrophicfactorlevels[J].Hippocampus,2016,24(4):415-423

[3]韩刚,王以东,曹羽,等.直肠癌腹腔镜与开腹根治术的近远期疗效及安全性评估[J].现代生物医学进展,2016,13(8):1511-1513+1553

HanGang,WangYi-dong,CaoYu,etal.StudyontheShort-TermandLong-TermEffectandSafetyofLaparoscopicVersusOpenRadicalResectionforRectalCancer[J].ProgressinModernBiomedicine,2016,13(8):1511-1513+1553

英文模版()

AssociationStudyofHumanSerotonin5-HTR2AgenePolymorphismswithAlcoholDependenceinYunnanYiopulation*

YANGHao1,ZHONGShu-rong1,ZHAOLi-juan1,LIJin-hua1,ZENGXi-ran2,JINGQiang1△

(1KunmingMedicalUniversity,Kunming,Yunnan,650000,China,2UniversityofYork,Heslington,York,YO105DD,UK)

ABSTRACTObjective:Toinvestigatetherelationshipbetweenalcoholdependenceandpolymorphismofserotoninreceptor2a(5-HTR2A)geners6311inYunnanYipopulation.Methods:ThePCR-RFLPwasusedtoanalyzegeicpolymorphismof5-HT2ARgene,thegenotypeandgenefrequenciesin330controlgroupsand110ADgroups.Results:Twoallelesofthe5-HT2ARgeneweredetectedin440samples:allelesAandG,includingthreegenotypes:AA,AGandGG.Genotypefrequencywas30%,63.6%and6.4%inADgroups,and38.5%,55.8%and5.8%incontrolgroups,respectively.Conclusion:TherewasnosignificantassociationforHTR2Ageners6311betweenADgroupandcontrolgroupinYunnanYiPopulation.

Keywords:Alcoholdependence,HTR2A,Polymorphism,YunnanYipopulation

ChineseLibraryClassification(CLC):R394.3Documentcode:A

ArticleID:1673-6273

Introduction

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1MaterialsandMethods

Readersmustbeabletoreproduceyourresults,evaluatethevalidityofyourconclusionsandthesoundnessofyourmethods,andfollowthelogicinthispaper.

1.1Materials

详细说明研究对象(subject),包括:研究人员(subjects),性别(gender),年龄(age),物种(species),品种(breed),生理状态(physiologicalstate),微生物要说明菌株(strain),血

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